interferENZY

A web-based tool for enzymatic assay validation and standardized kinetic analysis.

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Instructions

  1. Download the spreadsheet dataset template.
  2. Fill in as indicated (see the included notations for reference).
  3. Save as Tab-delimited Text (*.txt). See a ready-to-use pre-filled example here.
  4. Upload the resulting *.txt file to the form below and press submit.


Dataset Requirements

  • The order by which S0 values are provided must match the order of the time-product/substrate progress curves in the data file;
  • Dataset must comprise reaction progress curves for fixed enzyme concentration E0 and varying substrate concentration S0 (minimum of 5 different values of S0);
  • Reaction progress curves and provided values of initial substrate concentration must be calibrated to units of molar concentration;
  • Product generation and substrate decay progress curves can be used;
  • Continuous or stopped assays can be used (minimum of two reaction points);
  • Reaction progress curves with different number of points can be included in the same dataset.